Dayton Petibone Ph.D.
Biologist — Genetic and Molecular Toxicology
Dayton Petibone, Ph.D.
(870) 543-7121
NCTRResearch@fda.hhs.gov
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About | Publications | Lab Members
Background
Dayton Petibone majored in biology with a minor in chemistry while earning a B.S. degree at Northern Michigan University in Marquette, Michigan. He continued to pursue a M.S. research degree at Northern Michigan University, which focused on identifying DNA response elements involved in hormonal regulation of insect cuticle protein gene expression. Dayton conducted Ph.D. work at the University of Arkansas at Little Rock, Arkansas. His Ph.D. research concentrated on understanding the effect tumor suppressor p53 functional status has on cytotoxicity and genotoxicity in human B-lymphoblastoid cells exposed to an oxidized graphene nanomaterial.
Dayton worked for many productive years in the Department of Biological Sciences at Wayne State University in Detroit, Michigan. At Wayne State, he participated in or supervised studies utilizing classical and molecular cytogenetic techniques. These studies included retrospective radiation biodosimetry, developing methods for applying human fluorescence in situ hybridization (FISH) probes to analysis of rhesus macaque chromosomes, and evaluating methylphenidate induced genotoxicity, among other research efforts. He was also involved in training and working with graduate students, undergraduate students, and visiting scientists conducting a variety of genotoxicity studies and gene expression experiments designed to identify biomarkers for radiation dosimetry. Since 2009, Dayton has worked as a biologist at the National Center for Toxicological Research (NCTR) in the Division of Genetic and Molecular Toxicology (DGMT). His research in DGMT involves investigating p53 function in the genotoxicity induced by agents of interest to the FDA. In addition, his research efforts include the development, modernization, and validation of in vitro gene mutation tests for higher throughput assessments relevant to both human exposures and to carcinogenesis. Recently, Dr. Petibone was awarded the 2016 NCTR Excellence in Laboratory Science Award for participating in a workgroup that established human whole genome sequencing at NCTR and characterized the genome of cell lines with different p53 functional status that are commonly used in genetic toxicology. He also received the 2016 FDA Group Recognition Award for participating in characterization of a novel gene mutation assay for regulatory safety assessments.
Research Interests
Dr. Petibone specializes in developing and applying molecular FISH techniques to assessing the ability of potential genotoxicants to induce structural chromosome damage.
Recently, Dr. Petibone has undertaken research to advance the development, modernization, and validation of existing gene mutation assays specifically for high throughput analysis, and the innovation of new gene mutation assays that are relevant to human cancer.
Currently, Dr. Petibone is a Principal Investigator or Co-Investigator on several scientific projects funded through FDA Challenge Grants, including two Medical Countermeasures initiative (MCMi), Perinatal Health Center for Excellence (PHCE), and Office of Women’s Health (OWH) grants. Dr. Petibone provides guidance and collaborates with other scientists—from the Center for Biologics Evaluation and Research, the Center for Devices and Radiological Health, and industry—on the development and evaluation of microphysiological systems as alternative models for traditional toxicity techniques and as models of viral infection.
Professional Societies/National and International Groups
Environmental Mutagen and Genomics Society
Member
2004 – Present
Society of Toxicology
Member
2019 – Present
Select Publications
p53-Competent Cells and p53-Deficient Cells Display Different Susceptibility to Oxygen Functionalized Graphene Cytotoxicity and Genotoxicity.
Petibone D.M., Mustafa T., Ding W., Bourdo S., Lafont A., Watanabe F., Casciano D., Morris S.M., Dobrovolsky V., and Biris A.
Journal of Applied Toxicology. 2017, doi: 10.1002/jat.3472.
In Vivo Rat T-Lymphocyte Pig-A Assay: Detection and Expansion of Cells Deficient in the GPI-Anchored CD48 Surface Marker for Analysis of Mutation in the Endogenous Pig-A Gene.
Dobrovolsky V., Revollo J., Petibone D., and Heflich R.
Methods Mol Biol. 2017, 1641:143-160.
Autophagy Function and Its Relationship to Pathology, Clinical Applications, Drug Metabolism, and Toxicity.
Petibone D.M., Majeed W., and Casciano D.A.
Journal of Applied Toxicology. 2017, 37(1):23-37.
The Role of Surface Chemistry in the Cytotoxicity Profile of Graphene.
Majeed W., Bourdo S., Petibone D., Saini V., Vang K.B., Nima Z.A., Alghazali K.M., Darrigues E., Ghosh A., Watanabe F., Casciano D., Ali S.F., and Biris A.S.
J Appl Toxicol. 2017, 37 (4): 462-470.
Whole Genome and Normalized mRNA Sequencing Reveal Genetic Status of TK6, WTK1, and NH32 Human B-Lymphoblastoid Cell Lines.
Revollo J., Petibone D.M., McKinzie P., Knox B., Morris S.M., Ning B., Dobrovolsky V.N.
Mutat Res Genet Toxicol Environ Mutagen. 2016, 795:60-9.
Confirmation of Pig-a Mutation in Flow Cytometry-Identified CD48-Deficient T-Lymphocytes Derived from Spleens of ENU-Treated F344 Rats.
Revollo J., Pearce M.G., Petibone D.M., Mittelstaedt R.A., and Dobrovolsky V.N.
Mutagenesis. 2015, 30(3):315-24.
Autophagy Function and its Relationship to Pathology, Clinical Applications, Drug Metabolism, and Toxicity.
Petibone D., Majeed W., and Casciano D.
Journal of Applied Toxicology. 2016, 37 (1): 23-37
Whole Genome and Normalized Mrna Sequencing Reveal Genetic Status of TK6, WTK1, and NH32 Human B-Lymphoblastoid Cell Lines.
Revollo J., Petibone D., McKinzie P., Knox B., Morris S.M., Ning B., and Dobrovolsky V.N.
Mutat Res Genet Toxicol Environ Mutagen. 2016, 795:60-9.
Confirmation of Pig-A Mutation in Flow Cytometry-Identified CD48-Deficient T-Lymphocytes Derived from Spleens of ENU-Treated F344 Rats.
Revollo J., Pearce M., Petibone D., Mittelstaedt R., and Dobrovolsky V.
Mutagenesis. 2015, 30 (3): 315-324.
Chromosome Painting of Mouse Peripheral Blood and Spleen Tissues. (Book Chapter)
Petibone D., Tucker J., and Morris S.
In Gaivão I. and Sierra L.M. (Eds.) Genotoxicity and DNA Repair: A Practical Approach.
Methods in Pharmacology and Toxicology. 2014, 141-158.
p53 Alters the Biologically Effective Dose, Cytotoxicity, and Genotoxicity for Oxidized Graphene in Human Lymphoblastoid Cells.
Petibone D., Mustafa T., Ding W., Bourdo S., Lafont A., Watanabe F., Casciano D., Morris S., Dobrovolsky V., and Biris A.
J Toxicology. 2014 Jun 20.
Toxicity and Efficacy of Carbon Nanotubes and Graphene: The Utility of Carbon-Based Nanoparticles in Nanomedicine.
Zhang Y., Petibone D., Xu Y., Mahmood M., Karmakar A., Casciano D., Ali S., and Biris A.S.
Drug Metab Rev. 2014, 46(2):232-46.
In Vivo Genotoxicity of Furan in F344 Rats at Cancer Bioassay Doses.
Ding W., Petibone D., Latendresse J.R., Pearce M.G., Muskhelishvili L., White G.A., Chang C.-W., Mittelstaedt R.A., Shaddock J.G., McDaniel L.P., Doerge D.R., Morris S.M., Chen J., Manjanatha M.G., Aidoo A., and Heflich R.H.
Toxicology and Applied Pharmacology. 2012, 261(2): 164-171.
The Genetic Toxicity of Methylphenidate: A Review of the Current Literature.
Morris S.M., Petibone D., Lin W.-J., Chen J.J., Vitiello B., Witt K.L., and Mattison D.R.
Journal Of Applied Toxicology. 2012, 32(10): 756-764.
Evaluation of P53 Genotype on Gene Expression in the Testis, Liver and Heart from Male C57BL/6 Mice.
Petibone D.M., Kulkarnia R., Changb C.W., Chen J.J., and Morrisa S.M.
Transgenic Res. 2012, 21:257–263.
Pubertal Delay in Male Non-Human Primates (Macaca mulatta) Treated with Methylphenidate.
Mattison D.R., Plant T.M., Lin H.-M., Chen H.-C., Chen J.J., Twaddle N.C., Doerge D., Slikker W. Jr., Patton R.E., Hotchkiss C.E., Callicott R.J., Schrader S.M., Turner T.W., Kesner J.S., Vitiello B., Petibone D.M., and Morris S.M.
Proc Natl Acad Sci U S A. 2011, 108(39):16301-6.
Effect of P53 Genotype on Gene Expression and DNA Adducts in ENU-Exposed Mice.
Kulkarni R., Petibone D., Chang C.-W., Chen J.J., Tolleson W.H., Melchior W.B. Jr., Churchwell M.I., Beland F.A., and Morris S.M.
General, Applied and Systems Toxicology. 2011.
Oligonucleotide Immobilization using 10-(carbomethoxy)Decyl-Dimethylchlorosilane for mRNA Isolation and cDNA Synthesis on a Microfluidic Chip.
Hughes-Chinkhota C.N., Banda M., Smolinski J.M., Thomas R.A., Petibone D.M., Tucker J.D., and Auner G.W.
Sensors and Actuators B: Chemical. 2011, 155 (2): 437-45.
Cytogenetic Assessment of Methylphenidate Treatment in Pediatric Patients Treated for Attention Deficit Hyperactivity Disorder.
Tucker J.D., Suter W., Petibone D., Thomas R.A., Bailey N.L., Zhou Y., Zhao Y., Muniz R., and Kumar V.
Mutation Research. 2009, 677(1-2):53-8.
The Genetic Toxicology of Methylphenidate Hydrochloride in Non-Human Primates.
Morris S.M., Dobrovolsky V.N., Shaddock J.G., Mittelstaedt R.A., Bishop M.E., Manjanatha M.G., Shelton S.D., Doerge D.R., Twaddle N.C., Chen J.J., Lin C.J., Paule M.G., Slikker W. Jr., Hotchkiss C.E., Petibone D., Tucker J.D., and Mattison D.R.
Mutation Research. 2009, 673(1):59-66.
Routine Diagnostic X-ray Examinations and Increased Frequency of Chromosome Translocations Among United States Radiologic Technologists.
Sigurdson A.J., Bhatti P., Preston D.L., Doody M.M., Kampa D., Alexander B.H., Petibone D., Yong L.C., Edwards A.A., and Tucker J.D.
Cancer Research. 2008, 68 (21): 8825-31.
Technique for Culturing Macaca mulatta Peripheral Blood Lymphocytes for Fluorescence in Situ Hybridization of Whole Chromosome Paints.
Petibone D.M., Morris S.M., Hotchkiss C.E., Mattison D.R., and Tucker J.D.
Mutation Research. 2008, 653:76-81.
Lab Members
Sharon Shelton
Biologist
- Contact Information
- Dayton Petibone
- (870) 543-7121
- Expertise
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ExpertiseApproachDomainTechnology & DisciplineToxicology