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In this section: Laboratory Methods (Food)

BAM Media M147: Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS) Agar

Media Index for BAM

January 2001

Yeast extract	5 g	Oxgall	5 g
Peptone	10 g	NaCl	10 g
Sucrose	20 g	Ferric citrate	1 g
Sodium thiosulfate 5H₂O	10 g	Bromthymol blue	0.04 g
Sodium citrate 2H₂O	10 g	Thymol blue	0.04 g
Sodium cholate	3 g	Agar	15 g
Distilled water			1 liter

Prepare in flask at least 3 times larger than required volume of medium. Add ingredients to warm distilled water and heat to dissolve. Bring just to boil, and immediately remove from heat. DO NOT AUTOCLAVE! Cool to 50°C and pour into petri dishes. Dry the plates overnight or at 37-45°C before use.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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