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  5. BAM Chapter 4A: Diarrheagenic Escherichia coli
  1. Laboratory Methods (Food)

BAM Chapter 4A: Diarrheagenic Escherichia coli

Bacteriological Analytical Manual (BAM) Main Page

Authors

Peter Feng (ret.), Stephen D. Weagant (ret.), Karen Jinneman

Editions

Current Edition 

Previous Editions 

Revision History

  • July 2020: Update to section R.1.c for use of instrumental methods to screen for E. coli and correction to Table 5.
  • October 2018: Sec. R. #2. Procedure for confirmation of non-O157 STEC isolates has been clarified with instructions to perform WGS and PFGE on all STEC isolates.
  • October 2017: Sec. Q.1.c. The 5P PCR and LIB 3811 methods used for confirming O157:H7 isolates have been deleted and archived. Also, instruction were added to do PFGE and WGS on all O157:H7 isolates.
  • October 2017: Additional instructions and cautionary statements have been added to Sec. R.1.c on isolation of STEC. Specifically, the number of colony picks has been increased and with specific instructions.
  • August 2016: The Lightcycler method has been archived and replaced with the AB 7500 Fast platform. Extensive Modifications to Section K: Screening method for E. coli Serotype O157:H7/STEC from Foods; Section L: Equipment and Materials; Section M: Media and Reagents, Section N: Sample Preparation and Enrichment Procedure, and Section O: Real-time PCR Screening.
  • November 2015: Section R: The 13-plex Luminex suspension array: STEC Molecular Serotyping and Virulence Profiling Protocol replaces the 11-plex STEC Molecular Serotyping Protocol, which will be archived, when reagents for the 13-plex assay becomes available. The new Luminex based suspension array can now identify 11 clinically relevant STEC O serogroups and detect two STEC adherence factor genes: eae and aggR.
  • July 2014: Section N: A new sample preparation for leafy produce (except cilantro and parsley) has been added; the sample preparation for cilantro and parsley has been modified.
  • July, 2013: Section R: STEC Molecular Serotyping Protocol: Luminex-based suspension array to identify 11 STEC O serogroups has been added.
  • December 2012: Section R: The testing for non-O157 STEC has been changed from optional to required. All samples that are PCR positive for Stx1 and Stx2 but negative for uidA, must be followed up with additional testing to isolate the STEC organism. In Section R.1.a. the SHIBAM agar has been added to facilitate the isolation of STEC from enrichment samples.
  • February 2011: Section M: Addition of primers in Table 2; Section Q: addition of 5P Multiplex PCR for confirmation of O157:H7 isolates
  • July 2009: Section M modified to include the real-time PCR screening method
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