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In this section: Laboratory Methods (Food)

BAM Media M155: Trypticase (Tryptic) Soy Broth (TSB) with Glycerol

Media Index for BAM

January 2001

Trypticase peptone (tryptone)	17 g
Phytone peptone (soytone)	3 g
NaCl	15 g
K₂HPO₄	2.5 g
Glycerol	240 ml
Distilled water	760 ml

Suspend ingredients in distilled water and heat to dissolve. Dispense into bottles or vials. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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