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In this section: Laboratory Methods (Food)

BAM Media M192c: Modified Buffered Peptone Water (mBPW)

Media Index for BAM

May 2024

Peptone	10.0 g
Sodium Chloride	5.0 g
Disodium Phosphate (Na2HPO4)	7.0 g
Monopotassium Phosphate (KH2PO4)	3.0 g
Distilled water	1000 ml

Autoclave at 121°C for 15 min. Final pH 7.2±0.2 at 25 °C

Note: Commercial Modified Buffered Peptone Water (Hardy Diagnostics CRITERIONTM; Catalog number C6351) or any other product which provides the same formulation as above may be substituted for the above formulation; Commercial Buffered Peptone Water (BPW; BD catalog number 218105) may be substituted for the above formulation by add 3.5 g of Na₂HPO₄ and 1.5 g of KH₂PO₄ to 20 g commercial BPW in 1000 ml distilled water.

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