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In this section: Laboratory Methods (Food)

BAM Media M3: Acetate Agar

Media Index for BAM

January 2001

Sodium acetate	2 g
NaCl	5 g
MgSO₄ (anhydrous)	0.2 g
Ammonium phosphate	1 g
K₂HPO₄	1 g
Bromthymol blue	0.08 g
Agar	20 g
Distilled water	1 liter

Add all ingredients except MgSO₄ to 1 liter distilled water. Heat to boiling with stirring. Add MgSO₄ and adjust pH. Dispense 8 ml portions into 16 × 150 mm tubes. Autoclave 15 min at 121°C. Incline tubes to obtain 5 cm slant. Final pH, 6.7.

NOTE: This medium contains a slightly smaller concentration of acetate than the formula recommended in Ewing (1986).

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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