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In this section: Laboratory Methods (Food)

BAM Media M49: EC Broth

Media Index for BAM

January 2001

Trypticase or tryptose	20 g
Bile salts No. 3	1.5 g
Lactose	5 g
K₂HPO₄	4 g
KH₂PO₄	1.5 g
NaCl	5 g
Distilled water	1 liter

Distribute 8 ml portions to 16 × 150 mm test tubes containing inverted 10 × 75 mm fermentation tubes. Autoclave 15 min at 121°C. Final pH, 6.9 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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