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In this section: Laboratory Methods (Food)

BAM Media M89: Lysine Iron Agar (Edwards and Fife)

Media Index for BAM

January 2001

Gelysate or peptone	5 g
Yeast extract	3 g
Glucose	1 g
L-Lysine hydrochloride	10 g
Ferric ammonium citrate	0.5 g
Sodium thiosulfate (anhydrous)	0.04 g
Bromcresol purple	0.02 g
Agar	15 g
Distilled water	1 liter

Heat to dissolve ingredients. Dispense 4 ml portions into 13 × 100 mm screw-cap tubes. Autoclave 12 min at 121°C. Let solidify in slanted position to form 4 cm butts and 2.5 cm slants. Final pH, 6.7 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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