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In this section: Laboratory Methods (Food)

BAM R30: Giemsa Stain

Reagents Index for BAM

January 2001

Giemsa stain (Matheson Coleman & Bell, Norwood, OH 45212)	1 g
Glycerol	66 ml
Methanol (absolute)	66 ml

Distilled stain in glycerol by heating 1.5-2.0 h at 55-60°C. Add methanol. Store stain in tightly stoppered bottle at 22°C for at least 2 weeks. Dilute stock solution with distilled water (1+9) before use.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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