An official website of the United States government Here’s how you know

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

On Oct. 1, 2024, the FDA began implementing a reorganization impacting many parts of the agency. We are in the process of updating FDA.gov content to reflect these changes.

In this section: Laboratory Methods (Food)

BAM Media M167: Tryptose Broth and Agar (for serology)

Media Index for BAM

January 2001

Difco Bacto tryptose	20 g
NaCl	5 g
Dextrose	1 g
Agar	15 g
Distilled water	1 liter

Weigh ingredients, add water, and mix. Autoclave 15 min at 121°C. Swirl to disperse molten agar. Final pH, 7.2 ± 0.2.

For broth, omit agar from formulation.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Top