BAM Media M17: Baird-Parker Medium, pH 7.0
January 2001
Basal medium
| Tryptone | 10 g |
|---|---|
| Beef extract | 5 g |
| Yeast extract | 1 g |
| Sodium pyruvate | 10 g |
| Glycine | 12 g |
| Lithium chloride·6H2O | 5 g |
| Agar | 20 g |
Autoclave 15 min at 121°C. Final pH, 7.0 ± 0.2. If desired for immediate use, maintain melted medium at 48-50°C before adding enrichment. Otherwise, store solidified medium at 4 ± 1°C up to 1 month. Melt medium before use.
Enrichment
Bacto EY tellurite enrichment.
Complete medium
Aseptically add 5 ml prewarmed (45-50°C) Bacto EY tellurite enrichment to 95 ml melted base. Mix well (avoiding bubbles) and pour 15-18 ml portions into sterile 15 × 100 mm petri dishes. The medium must be densely opaque. Dry plates before use. Store prepared plates at 20-25°C for up to 5 days. See Official Methods of Analysis of AOAC International, 15th Edition (1990), p. 429, for more information.
| Storage | 20-25°C | 5 days |
|---|
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998. Revised by rim 2000-03-28