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In this section: Laboratory Methods (Food)

BAM Media M176: Vogel-Johnson (VJ) Agar

Media Index for BAM

January 2001

Trypticase	10 g
Yeast extract	5 g
Mannitol	10 g
K₂HPO₄	5 g
Lithium chloride	5 g
Glycine	10 g
Phenol red	0.025 g
Agar	15 g
Distilled water	1 liter

Heat with agitation to dissolve agar. Autoclave 15 min at 121°C. Cool to 50°C. Add 20 ml Chapman tellurite solution, 1%, commercially available from Difco Laboratories, Detroit, MI. Mix and pour into plates. Final pH, 7.2 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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