BAM R12: Catalase Test
January 2001
Pour 1 ml 3% hydrogen peroxide over growth on slant culture. Gas bubbles indicate positive test. Alternatively, emulsify colony in l drop 3% hydrogen peroxide on glass slide. Immediate bubbling is positive catalase test. If colony is taken from blood agar plate, any carry-over of red blood cells can give false-positive reaction.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998