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In this section: Laboratory Methods (Food)

BAM R31: Glycerin-Salt Solution (Buffered)

Reagents Index for BAM

January 2001

Glycerin (reagent grade)	100 ml
K₂HPO₄ (anhydrous)	12.4 g
KH₂PO₄ (anhydrous)	4 g
NaCl	4.2 g
Distilled water	900 ml

Distilled NaCl and bring volume to 900 ml with water. Add glycerin and phosphates. Adjust pH to 7.2. Autoclave 15 min at 121°C. For double strength (20%) glycerin solution, use 200 ml glycerin and 800 ml distilled water.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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