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In this section: Laboratory Methods (Food)

BAM R77: Standard Saline Citrate (SSC) Solution (20%)

Reagents Index for BAM

January 2001

NaCl (reagent grade)	175.3 g
Sodium citrate	88.2 g

Dissolve in 800 ml deionized water and adjust to pH 7 with 10 N NaOH. Bring volume to 1 liter.

6X SSC

NaCl (reagent grade)	52.6 g
Sodium citrate	26.5 g

Dissolve in 800 ml deionized water and adjust to pH 7 with 10 N NaOH. Bring volume to 1 liter.

3X SSC

6× SSC	500 ml
Deionized water	500 ml

2X SSC

6× SSC	333 ml
Deionized water	667 ml

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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